When you generate different emotions with your audience you need to ensure that you have proper transitions to guide your audience from one phase to the next emotional phase.
When you give various serious messages and want to make the transition to a more joyful atmosphere there needs to be some time in-between with good clear explanation / careful chosen words. If you don’t ensure a proper transition a joyful section (for example a funny video) that follows after a serious section, may appear to be totally inappropriate in the perspective of your audience. It may actually separate you from the audience as it could create a disconnect.
Therefore it is important to consider and have a good sense about the atmosphere / feeling of your audience. Ensure for proper transitions between the different emotional phases of your presentation.